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KMID : 0878220050170020064
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Journal of Sasang Constitutional Medicine
2005 Volume.17 No. 2 p.64 ~ p.73
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The Effects of Palmulgunja-tang(八物君子湯)Enzyme Activity on Cytochrome P450 Isozyme
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Ryu Jung-Man
Park Seong-Sik
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Abstract
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1.Objectives The purpose of this study was to investigate the effects of the enzyme activity of Palmulgunja-tang with administered orally solution on cytochrome P450 isozyme
2.Methods This study was carried on through following methods. We treated the rat with the {3-naphthoflavone (βNF) of 80mg/kg for 3 days i.p injection. Firstable, microsomal protein was separated and total intracellular protein test was done. Then GOT and GPT were measured and assay of cytochrome P450 IAI/2 enzyme activity was performed according to the method of EROD and MROD. (Ethoxyresorufin-O-deethylase(EROD) activity was used to measure cytochrome P450 lAI activity and methoxyresorufin O-demethylase(MROD) activity was used to measure cytochrome P450 lA2 activity. )
3.Results and Conclusions 1) PGT recovered the liver damage on βNFinducible CYP IAI/2 by pre-post and high-low condition. 2) At concentration of post-treated 50mg!kg of PGT, the inhibiting of [＼bciaNF]metabolites to liver of rat cytochrome P450 lAl was inhibited by 53.0% respectively. 3) PGT showed 36.0% inhibition of βNF-induced lA2 activity at the concentration post-treated 50mg/kg.
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KEYWORD
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Palmulgunja-tang, Cytochrome P450 isozyme, Enzyme activity
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